(4-nitrophenyl) (9E,12E)-octadeca-9,12-dienoate
CAS: 6562-50-1
Category: Organic Intermediates
| Purity | Research Grade ≥90%, High-Purity Grade ≥95%, Biochemical Grade ≥97% (for enzyme assays), Analytical |
| Molecular Formula | C₂₄H₃₅NO₄ |
| Molecular Weight | 401.54 |
| Appearance | Yellow to amber or pale yellow oily liquid or low-melting solid (depending on purity and temperature) |
| Packaging | 100mg/500mg/1g/5g/10g/25g/glass bottle or aluminum foil bottle (for research); 100g/500g/1kg/plastic bottle or PTFE-lined container; custom packaging for bulk orders |
| Testing Indicators | Appearance — Visual inspection (yellow to amber color); Identification — NMR (¹H, ¹³C), FTIR (characteristic ester C=O stretch ~1735–1750 cm⁻¹, nitrophenyl NO₂ stretches ~1520 cm⁻¹ and ~1350 cm⁻¹, alkene C=C stretch ~1650 cm⁻¹), or LC-MS/MS; Purity — HPLC (C18 column, UV detection at 254 nm, 280 nm, or 400 nm); Free 4-nitrophenol content — HPLC or UV-Vis spectrophotometry (absorbance at 400 nm in alkaline buffer); Water content — Karl Fischer titration; Peroxide value (oxidation status) — Iodometric titration; Acid value (free fatty acid content) — Titration; Refractive index — Refractometry; Density — Hydrometer or digital densitometer; Residual solvents — GC; Heavy metals — ICP-MS. |
| Storage Notes | Stored in a tightly sealed container in a cool, dry, well-ventilated area, protected from light, moisture, and heat; away from strong oxidizers, strong acids, strong bases, and reducing agents; store under inert atmosphere (nitrogen or argon) for long-term stability; keep container closed when not in use to prevent hydrolysis; store at -20°C (long-term) or 2–8°C (short-term) – the ester is susceptible to hydrolysis. |
| Lead Time | days |
Used as a research chemical in biochemical and enzymatic assays; as a chromogenic substrate for lipases, esterases, and phospholipases – enzymatic hydrolysis releases 4-nitrophenol (p-nitrophenol), which can be quantified spectrophotometrically at 400–410 nm (yellow color in alkaline conditions); as a model substrate for studying fatty acid ester hydrolysis and transesterification; in the development of high-throughput screening assays for enzyme inhibitors and activators; used in lipid metabolism research and food chemistry studies; the (9E,12E)-dienoate configuration represents the trans,trans isomer of linoleic acid (as opposed to the natural cis,cis isomer).